Dynamic response of ultrathin highly dense ZIF-8 nanofilms

Ultrathin ZIF-8 nanofilms are prepared by facile step-by-step dip coating. A critical withdrawal speed allows for films with a very uniform minimum thickness. The high refractive index of the films denotes the absence of mesopores. The dynamic response of the films to CO2 exposure resembles behaviour observed for nonequilibrium organic polymers

Alternativní zemědělství - informační bulletin č.7

Témata informačního bulletinu č.7 jsou: Vyhodnocení výnosů zemědělských plodin v roce 1991 u organicky hospodařících zemědělců svazu PRO-BIO. Nutnost moření osiva z hlediska alternativního zemědělství. Využití přirozených surovin v alternativním zemědělství. Mechanická regulace plevelů. Možnosti realizace alternativního zemědělství z hlediska ochrany rostlin. Regulace hraboše polního bez chemie. Úvod k připravované knize "Ekologická východiska zemědělství". Stáj s hlubokou podestýlkou pro dojnice z hlediska etologie a ekonomiky. Zelené hnojení. Organické vinohradnictví

Structure of the Herpes Simplex Virus Capsid: Peptide A862-H880 of the Major Capsid Protein Is Displayed on the Rim of the Capsomer Protrusions

AbstractThe herpes simplex virus-1 (HSV-1) capsid shell has 162 capsomers arranged on aT= 16 icosahedral lattice. The major capsid protein, VP5 (MW = 149,075) is the structural component of the capsomers. VP5 is an unusually large viral capsid protein and has been shown to consist of multiple domains. To study the conformation of VP5 as it is folded into capsid protomers, we identified the sequence recognized by a VP5-specific monoclonal antibody and localized the epitope on the capsid surface by cryoelectron microscopy and image reconstruction. The epitope of mAb 6F10 was mapped to residues 862–880 by immunoblotting experiments performed with (1) proteolytic fragments of VP5, (2) GST-fusion proteins containing VP5 domains, and (3) synthetic VP5 peptides. As visualized in a three-dimensional density map of 6F10-precipitated capsids, the antibody was found to bind at sites on the outer surface of the capsid just inside the openings of thetrans-capsomeric channels. We conclude that these sites are occupied by peptide 862–880 in the mature HSV-1 capsid

Clinical and humoral determinants of congestion in heart failure. potential role of adiponectin

Background: Some patients with heart failure (HF) are more prone to systemic congestion than others. The goal of this study was to identify clinical and humoral factors linked to congestion and its prognostic impact in HF patients. Methods: A total of 371 advanced HF patients underwent physical examination, echocardiography, right heart catheterization, blood samplings, and Minnesota Living with HF Questionnaire. Subjects were followed-up for adverse events (death, urgent transplantation, or assist device implantation without heart transplantation). Results: Thirty-one percent of patients were classified as prone to congestion. During a median follow-up of 1,093 days, 159 (43%) patients had an adverse event. In the Cox analysis, the congestion-prone (CP) status was associated with a 43% higher event risk. The CP status was strongly (p ? 0.001) associated with body weight loss, right ventricular dysfunction (RVD), dilated inferior vena cava (IVC), diuretics, and beta-blockers prescription and the majority of tested hormones in the univariate analysis. In the multivariate analysis, the only independent variables associated with the CP status were adiponectin, albumin, IVC diameter, and RVD. Adiponectin by itself was predictive of adverse events. In a multivariate model, CP status was no longer predictive of adverse events, in contrast to adiponectin. Conclusions: CP patients experienced more severe symptoms and had shorter survival. Potential role of adiponectin, a new independent predictor of CP status, should be further examined

Thermal characterization of Montmorillonite clays saturated with various cations

Emanation thermal analysis (ETA), thermogravimetry and high temperature XRD were used to characterize the thermal behavior during dehydration of natural Na montmorillonite (Upton Wyoming, USA) and homoionic montmorillonite (MMT) samples saturated with different cations, i.e. Li+, Cs+, NH 4 +, Mg2+ and Al3+. ETA results characterized radon mobility and microstructure changes that accompanied the mass loss of the samples due to dehydration on heating in air. A collapse of interlayer space between the silicate sheets after water release from the MMT samples was characterized by a decrease of the radon release rate, ΔE. Decreases in c-axis basal spacing (d 001) values determined from XRD patterns for the different montmorillonite samples follow the sequence: Mg−MMT>Al−MMT>Li−MMT>Na−MMT>NH4−MMT>Cs−MMT The decrease of the radon release rate (ΔE) determined by ETA that characterized microstructure changes due to collapse of interlayer space corresponded well to differences in the c-axis basal spacing (Δd 001) values determined from the XRD patterns before and after samples dehydration.Peer Reviewe

High-pressure CO2/CH4 separation of Zr-MOFs based mixed matrix membranes

The gas separation properties of 6FDA-DAM mixed matrix membranes (MMMs) with three types of zirconium-based metal organic framework nanoparticles (MOF NPs, ca. 40 nm) have been investigated up to 20 bar. Both NPs preparation and MMMs development were presented in an earlier publication that reported outstanding CO2/CH4 separation performances (50:50 vol% CO2/CH4 feed at 2 bar pressure difference, 35 °C) and this subsequent study is to demonstrate its usefulness to the natural gas separation application. In the current work, CO2/CH4 separation has been investigated at high pressure (2–20 bar feed pressure) with different CO2 content in the feed (10–50 vol%) in the temperature range 35–55 °C. Moreover, the plasticization, competitive sorption effects, and separation of the acid gas hydrogen sulfide (H2S) have been investigated in a ternary feed mixture of CO2:H2S:CH4 (vol% ratio of 30:5:65) at 20 bar and 35 °C. The incorporation of the Zr-MOFs in 6FDA-DAM enhances both CO2 permeability and CO2/CH4 selectivity of this polymer. These MMMs exhibit high stability under separation conditions relevant to an actual natural gas sweetening process. The presence of H2S does not induce plasticization but increases the total acid gas permeability, acid gas/CH4 selectivity and only causes reversible competitive sorption. The overall study suggests a large potential for 6FDA-DAM Zr-MOF MMMs to be applied in natural gas sweetening, with good performance and stability under the relevant process conditions

Thermal characterization of Montmorillonite clays saturated with various cations

Emanation thermal analysis (ETA), thermogravimetry and high temperature XRD were used to characterize the thermal behavior during dehydration of natural Na montmorillonite (Upton Wyoming, USA) and homoionic montmorillonite (MMT) samples saturated with different cations, i.e. Li+, Cs+, NH 4 +, Mg2+ and Al3+. ETA results characterized radon mobility and microstructure changes that accompanied the mass loss of the samples due to dehydration on heating in air. A collapse of interlayer space between the silicate sheets after water release from the MMT samples was characterized by a decrease of the radon release rate, ΔE. Decreases in c-axis basal spacing (d 001) values determined from XRD patterns for the different montmorillonite samples follow the sequence: Mg−MMT>Al−MMT>Li−MMT>Na−MMT>NH4−MMT>Cs−MMT The decrease of the radon release rate (ΔE) determined by ETA that characterized microstructure changes due to collapse of interlayer space corresponded well to differences in the c-axis basal spacing (Δd 001) values determined from the XRD patterns before and after samples dehydration.Ministry of Education of Czech Republic LA–292España Ministerio de educación MAT2004-0264

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments. CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for evaluating qPCR experiments. Included is a checklist to accompany the initial submission of a manuscript to the publisher. By providing all relevant experimental conditions and assay characteristics, reviewers can assess the validity of the protocols used. Full disclosure of all reagents, sequences, and analysis methods is necessary to enable other investigators to reproduce results. MIQE details should be published either in abbreviated form or as an online supplement. SUMMARY: Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results